Some Factors Affecting the Hill Reaction Activity in Cotton Chloroplasts
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چکیده
منابع مشابه
Some factors affecting the Hill reaction activity in cotton chloroplasts.
A method of plant culture was developed for growing large leaves of glandless cotton on single stems. Chloroplasts isolated from these leaves actively reduced ferricyanide when assayed for the Hill reaction. Hill reaction activity increased 133% when the 0.5 m sucrose isolation medium was replaced with 10% (w/v) polyethylene glycol, both buffered at pH 7.6. The presence of 2 or 5% (w/v) bovine ...
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SimnlIrv(11'. 1lii order to avoid the l)roblelll of ani uncouplinig effect in stimiiulatioln of potential Hill reaction activity durinig extractioln with heptane, Hill reaction activity was measured with uncouple(d spinach chloroplasts. TI'he reactions were also runi ulnder aerobic coniditionis. Under these conditions a correctioni for a 3(p-chloroph'enyl) -1. 1-dimethylurea insensitive, photos...
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In order to avoid the problem of an uncoupling effect in stimulation of potential Hill reaction activity during extraction with heptane. Hill reaction activity was measured with uncoupled spinach chloroplasts. The reactions were also run under aerobic conditions. Under these conditions a correction for a 3-(p-chlorophenyl)-1, 1-dimethylurea insensitive, photostimulated O(2) uptake reaction is n...
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Light- and HCO(3) (-)-saturated (10 millimolar) rates of O(2) evolution (120 to 220 micromoles O(2) per milligram chlorophyll per hour), obtained with intact spinach chloroplasts, are decreased up to 3-fold by changes in assay conditions such as omission of catalase from the medium, the use of high (>/=1 millimolar) inorganic phosphate, inclusion of NO(2) (-) as an electron acceptor, or bright ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1970
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.45.4.465